• CUSTOM MODEL GENERATION SERVICES

    CRISPR-Cas9? ???? ??? ??? ???? ??

    ????? CRISPR-Cas9 ??? ???? ?? 4,000? ??? ??? ??? ??? ???? ???, ??? ?? ??? ?? ?? ?????. CRISPR-Cas9 ??? ??? ?? ?? ???? ??? ?? ???, ???(KO) ???, ??? ???(cKO) ???, ??(KI) ??? ??? ??? ? ????. 

    CRISPR-Cas9 ??? ALEN, ZFN, ES? ?? ?? ??? ?? ???? ???? ??? ?????.


    Advantages:

    • ???: ??? ??(random insertion)?? ??? ???(no serial recombination)? ?? ??? ??

    • ???: ????? ???? ?? ???? ???? ??? ?? ??? ??

    • Multi-modification: ?? ?? loci, gene, large fragment? ??? ? ? ??

    • ??? ??? ??: C57BL/6J, C57BL/6N, BALB/c, FVB, NOD/LTJ, NCG ? ??? ??? ???? ??? ?? ?? ??


    ?????? ?? ??? ??? ?? ??? ??? ?? ??

    ??????(Embryonic Stem Cell, ES) ?? ???(Homologous Recombination, HR) ??? ?? DNA? ES?? ??? ?? ???? ??? ?? ?????. ??? ??? ?? ??? ????? ??? DNA? ??? CRISPR-Cas9 ??? ??? ??? ???? ??? ??? ?? ??? ?????.


    ??:

    • ?? ???? ??? ??(Self-deletion of resistance genes)?? ?? ?? ??? 3?? ?? 

    • BAC ?? ??? 100-300kb? ??? ??(knock-in)? ? ?? 

    • 2005??? ??? ? ??? ???, 700? ??? ??? ? ??? ???? ????? ?????

    • 129S6/SvEvTac ? C57BL/6N ? ??? ??? ???? ??? ??? ? ??

    ????? ??? ??? ??? ??? ?? ??

    ????? ??? ?? ???(overexpressed) ?? ??? ???(conditionally overexpressed) ???? ??? ??? ???? ?? ?????. ????? ??? ??? ??? ?? ?? ?, ????(microinjection) ??? DNA? ??? ??? ??(pronucleus)? ?? ????? DNA? ??? ???? ???? ??(random insertion)???. ??(KI) ??? ???? ??, ??? ??? ?? ?? ? ???? ??? H11 ?? Rosa26 site? ???? ?????. 


    Comparison of transgenic technology and KI at the H11 or Rosa26 site

    Transgenic

    H11/Rosa26 Knockin

    F0 generation in as little as 8 weeks

    F1 generation in as little as 16 weeks

    Low technical difficulty

    High technical difficulty

    Different expression levels between founder lines, and expression levels over time (typically expression levels decrease)

    Same expression levels in all founder lines, and constant expression levels over time

    Random insertion site and number of copies

    Accurate insertion location and gene copy number by design

    Easy to alter or destroy the expression of other endogenous genes

    Does not affect expression other endogenous genes

    Poor genetic stability

    High genetic stability

    Large-scale work in establishing a stable line

    Produces a stable line directly for analysis


    TALK WITH OUR EXPERTS

    GemPharmatech is committed to protecting and respecting your privacy, and we'll only use your personal information to administer your account and to provide the products and services you requested from us. From time to time, we would like to contact you about our products and services, as well as other content that may be of interest to you. If you consent to us contacting you for this purpose, please click below to say how you would like us to contact you:

    In order to provide you with the content requested, we need to store and process your personal data. If you consent to us storing your personal data for this purpose, please click the checkbox below.

    You can unsubscribe from these communications at any time. For more information on how to unsubscribe, our privacy practices, and how we are committed to protecting and respecting your privacy, please review our Privacy Policy.